rabbit polyclonal anti-gcsf receptor  (Santa Cruz Biotechnology)

Journal: Cell reports

Article Title: Granulocyte colony stimulating factor promotes scarless tissue regeneration

doi: 10.1016/j.celrep.2024.114742

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The following primary antibodies were used: rabbit monoclonal anti-Arginase-1 (93668, Cell Signaling Technology), rabbit monoclonal anti-Ki-67 (9129, Cell Signaling), rabbit polyclonal anti-CD80 (8679, ProSci), rabbit polyclonal anti-Histone H3 (ab5103, Abcam), goat polyclonal anti-MPO (AF3667, R&D Systems), rabbit monoclonal anti-MRC1 (24595, Cell Signaling), rat monoclonal anti-F4/80 (ab6640, Abcam), rabbit polyclonal anti-Neutrophil Elastase (ab68672, Accam), mouse monoclonal anti-cytokeratin 14 (ab7800, Abscam), rabbit monoclonal anti keratin 6 (SAB5500131, Sigma), rabbit monoclonal anti-Phospho-Histone H3 (53348, Cell Signaling), rabbit monoclonal anti-CD31 (77699, Cell Signaling), rabbit monoclonal anti-F4/80 (70076, Cell Signaling), rabbit polyclonal anti-COX2 (ab15191, Abcam), rabbit anti-iNOS (2982, Cell Signaling), rabbit polyclonal anti-GCSFR (bs-2574R, Bioss) rabbit monoclonal anti-CD163 (68922, Cell Signaling) and rat anti-Ly6G (551459, BD Pharmingen).

Techniques: Recombinant, Conjugation Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: Molecular Medicine Reports

Article Title: Expression of granulocyte colony-stimulating factor 3 receptor in the spinal dorsal horn following spinal nerve ligation-induced neuropathic pain

doi: 10.3892/mmr.2017.6853

Figure Lengend Snippet: G-CSF expression increased in the spinal cord dorsal horn of rats following SNL. (A) The level of G-CSFR was measured in the lumbar dorsal horn by immunoblotting. Western blotting for G-CSF following SNL or sham surgery demonstrated that G-CSF expression was markedly increased in the ipsilateral dorsal horn when compared with the contralateral horn 14 days following surgery. (B) Reverse transcription-quantitative polymerase chain reaction analysis revealed that G-CSF mRNA expression significantly increased in the ipsilateral dorsal horn when compared with the contralateral horn following surgery. Data are presented as the mean ± standard error mean (n=7 rats/group). ***P<0.001 vs. the corresponding ipsilateral side (C) When compared to the (Ca) contralateral spinal dorsal horn, immunoreactive staining revealed that there was L5 SNL induced G-CSFR upregulation in the (Cb) ipsilateral lumbar spinal dorsal horn; G-CSFR staining was weakly observed in the contralateral dorsal horn (scale bar=40 mm). The images within the black rectangles are also shown at a higher magnification. G-CSFR immunoreactive staining in the (Cc) contralateral and (Cd) ipsilateral lumbar spinal dorsal horn, respectively (scale bars=20 mm). G-CSF, granulocyte-colony stimulating factor; G-CSFR, granulocyte-colony stimulating factor receptor; SNL, spinal nerve ligation; Con, contralateral dorsal horn; Ipsi, ipsilateral dorsal horn.

Article Snippet: For immunoblotting, membranes were incubated in 5% skim milk in TBS with 0.3% Triton X-100 for 1 h at room temperature to block non-specific binding and then probed with the following primary antibodies at 4°C overnight: Rabbit anti-G-CSF3 receptor (G-CSFR) polyclonal antibody (dilution, 1:500; cat. no. #bs-2574R; BIOSS), anti-β-actin (dilution, 1:5,000; cat. no. #A5316; Sigma; Merck KGaA), STAT3 (124H6) mouse monoclonal antibody (dilution, 1:1,000; cat. no. #9139; Cell Signaling Technology, Inc.), anti-p-STAT3 (Tyr705; M9C6) mouse (dilution, 1:200; cat. no. #4113; Cell Signaling, Inc.) and anti-capsaicin receptor (the TRPV1 antibody; dilution, 1:1,000; cat. no. MAB5568; EMD Millipore).

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Staining, Ligation

Journal: Molecular Medicine Reports

Article Title: Expression of granulocyte colony-stimulating factor 3 receptor in the spinal dorsal horn following spinal nerve ligation-induced neuropathic pain

doi: 10.3892/mmr.2017.6853

Figure Lengend Snippet: Double immunofluorescent staining of G-CSF (red) with NeuN, p-STAT3 and TRPV1 (green) in rats 14 days following SNL. (A) Double immunofluorescence staining for G-CSFR-positive cells in the ipsilateral spinal dorsal horn following SNL. Immunoreactive G-CSFR cells colocalized with NeuN, a neuronal marker of the superficial dorsal horn (indicated by white arrows). Double immunofluorescence demonstrated that G-CSFR colocalized with (B) p-STAT3 and (C) TRPV1 in the spinal dorsal horn following SNL treatment (indicated by arrows). Scale bar=20 µm. G-CSF, granulocyte-colony stimulating factor; G-CSFR, granulocyte-colony stimulating factor receptor; SNL, spinal nerve ligation; NeuN, neuronal nuclei; p-STAT3, phospho-signal transducer and activator of transcription 3; TRPV1, transient receptor potential cation channel subfamily V 1.

Article Snippet: For immunoblotting, membranes were incubated in 5% skim milk in TBS with 0.3% Triton X-100 for 1 h at room temperature to block non-specific binding and then probed with the following primary antibodies at 4°C overnight: Rabbit anti-G-CSF3 receptor (G-CSFR) polyclonal antibody (dilution, 1:500; cat. no. #bs-2574R; BIOSS), anti-β-actin (dilution, 1:5,000; cat. no. #A5316; Sigma; Merck KGaA), STAT3 (124H6) mouse monoclonal antibody (dilution, 1:1,000; cat. no. #9139; Cell Signaling Technology, Inc.), anti-p-STAT3 (Tyr705; M9C6) mouse (dilution, 1:200; cat. no. #4113; Cell Signaling, Inc.) and anti-capsaicin receptor (the TRPV1 antibody; dilution, 1:1,000; cat. no. MAB5568; EMD Millipore).

Techniques: Staining, Double Immunofluorescence Staining, Marker, Immunofluorescence, Ligation

Journal: Molecular Medicine Reports

Article Title: Expression of granulocyte colony-stimulating factor 3 receptor in the spinal dorsal horn following spinal nerve ligation-induced neuropathic pain

doi: 10.3892/mmr.2017.6853

Figure Lengend Snippet: G-CSF treatment increased G-CSFR expression, phosphorylation of STAT3 and TRPV1 expression in neurons. (A) Western blotting time course analysis of G-CSF, STAT3, p-STAT3, TRPV1 and b-actin following treatment with 50 µg/ml G-CSF in the SH-SY5Y neuronal cell line. Relative density was obtained by densitometry of the corresponding immunoblot data. Statistical differences between (B) G-CSFR, (C) p-STAT3 and (D) TRPV1 were determined by comparing values for β-actin at each lane. Data are expressed as optical densities and represent the mean ± standard error mean of three independent experiments. *P<0.05 and ***P<0.001 vs. 0 h. G-CSF, granulocyte-colony stimulating factor; G-CSFR, granulocyte-colony stimulating factor receptor; p-STAT3, phospho-signal transducer and activator of transcription 3; TRPV1, transient receptor potential cation channel subfamily V 1.

Article Snippet: For immunoblotting, membranes were incubated in 5% skim milk in TBS with 0.3% Triton X-100 for 1 h at room temperature to block non-specific binding and then probed with the following primary antibodies at 4°C overnight: Rabbit anti-G-CSF3 receptor (G-CSFR) polyclonal antibody (dilution, 1:500; cat. no. #bs-2574R; BIOSS), anti-β-actin (dilution, 1:5,000; cat. no. #A5316; Sigma; Merck KGaA), STAT3 (124H6) mouse monoclonal antibody (dilution, 1:1,000; cat. no. #9139; Cell Signaling Technology, Inc.), anti-p-STAT3 (Tyr705; M9C6) mouse (dilution, 1:200; cat. no. #4113; Cell Signaling, Inc.) and anti-capsaicin receptor (the TRPV1 antibody; dilution, 1:1,000; cat. no. MAB5568; EMD Millipore).

Techniques: Expressing, Western Blot